Peptide Reconstitution & Mixing Calculator
A precision engine for research chemicals. Convert vial milligrams into mcg per unit for U-100, U-40, and custom insulin syringes.
Popular Mixes
Mixing Parameters
Required Target Dose
Draw the liquid to the mark on your syringe.
Calculation Breakdown
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01
Convert vial size to micrograms:
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02
Determine mcg per mL of diluent:
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03
Divide by syringe capacity ( units) to find mcg/unit:
Understanding Peptide Reconstitution
Reconstitution is the process of adding a liquid (diluent) to a freeze-dried powder (lyophilized) so it can be measured for administration. In research environments, this requires absolute mathematical accuracy to ensure that the potency of the peptide matches the target research dose.
Selecting Your Diluent
Most researchers use Bacteriostatic Water (0.9% Benzyl Alcohol) because it inhibits bacterial growth, allowing the vial to be used for multiple draws over 28-30 days. Sterile saline is an alternative but lacks preservatives, making it suitable only for single-use applications.
U-100 Syringes
The standard 1mL syringe where 100 units equal 1mL. Most common in peptide research.
U-40 Syringes
Common in veterinary medicine where 40 units equal 1mL. Caution: Using U-100 math on a U-40 syringe results in a 2.5x overdose.
Peptide Stability Tips
- ✓ Avoid direct sunlight; UV rays can degrade peptide chains.
- ✓ Do not shake the vial vigorously. Gently swirl to mix.
- ✓ Always store reconstituted peptides in a refrigerator between 2°C and 8°C.
Standard Reconstitution Chart
Based on 5mg Vial + 2mL Diluent (U-100 Syringe)
| Desired Dose | Unit Pull | mL Volume | Doses Per Vial |
|---|---|---|---|
| 100 mcg | 4 Units | 0.04 mL | 50 Doses |
| 250 mcg | 10 Units | 0.10 mL | 20 Doses |
| 500 mcg | 20 Units | 0.20 mL | 10 Doses |
| 1000 mcg (1mg) | 40 Units | 0.40 mL | 5 Doses |